GOOD MANUFACTURING PRACTICE

"Good manufacturing practice" or "GMP" is part of a quality system covering the manufacture and testing of active pharmaceutical ingredients, diagnostics, foods, pharmaceutical products, and medical devices. GMPs are guidelines that outline the aspects of production and testing that can impact the quality of a product. Many countries have legislated that pharmaceutical and medical device companies must follow GMP procedures, and have created their own GMP guidelines that correspond with their legislation.

Although there are a number of them, all guidelines follow a few basic principles.

Manufacturing processes are clearly defined and controlled. All critical processes are validated to ensure consistency and compliance with specifications.Manufacturing processes are controlled, and any changes to the process are evaluated. Changes that have an impact on the quality of the drug are validated as necessary.

Instructions and procedures are written in clear and unambiguous language. (Good Documentation Practices)

Operators are trained to carry out and document procedures.

Records are made, manually or by instruments, during manufacture that demonstrate that all the steps required by the defined procedures and instructions were in fact taken and that the quantity and quality of the drug was as expected. Deviations are investigated and documented.

Records of manufacture (including distribution) that enable the complete history of a batch to be traced are retained in a comprehensible and accessible form.

The distribution of the drugs minimizes any risk to their quality.

A system is available for recalling any batch of drug from sale or supply.

Complaints about marketed drugs are examined, the causes of quality defects are investigated, and appropriate measures are taken with respect to the defective drugs and to prevent recurrence.

GMP guidelines are not prescriptive instructions on how to manufacture products. They are a series of general principles that must be observed during manufacturing. When a company is setting up its quality program and manufacturing process, there may be many ways it can fulfill GMP requirements. It is the company's responsibility to determine the most effective and efficient quality process.

GOOD LABORATORY PRACTICE

In the clinical and research arena, the phrase good laboratory practice or GLP generally refers to a system of management controls for laboratories and research organizations to ensure the consistency and reliability of results - as outlined in the Organisation for Economic Co-operation and Development (OECD) Principles of GLP and national regulations.GLP applies to non-clinical studies conducted for the assessment of the safety of chemicals to man, animals and the environment. The internationally accepted definition.Good Laboratory Practice (GLP) embodies a set of principles that provides a framework within which laboratory studies are planned, performed, monitored, recorded, reported and archived. These studies are undertaken to generate data by which the hazards and risks to users, consumers and third parties, including the environment, can be assessed for pharmaceuticals (only preclinical studies), agrochemicals, cosmetics, food additives, feed additives and contaminants, novel foods, biocides, detergents etc.... GLP helps assure regulatory authorities that the data submitted are a true reflection of the results obtained during the study and can therefore be relied upon when making risk/safety assessments.

GLP can become confused with the standards of laboratory safety - wearing appropriate gloves, glasses and clothing to handle materials safely.

GLP and the OECD

Following Decision C(97),186/Final of the OECD Council, data generated in the testing of chemicals in one OECD Member Country, in accordance with OECD Test Guidelines and the Principles of GLP are accepted in all other OECD Member Countries.

Definition

GLP is a quality system concerned with the organisational processing process and conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, archived and reported.

GLP ensures the quality, integrity, and reliability of safety data.

GLP principles include

1.Organization and Personnel

   Management-Responsibilities

   Sponsor-Responsibilities

   Study Director-Responsibilities

   Principle Investigator-Responsibilities

   Study Personnel-Responsibilities

2.Quality assurance program

   Quality Assurance Personnel

3.Facilities

   Test System Facilities

   Facilities for Test and Reference Items

4.Equipments, reagents and Materials

5.Test systems

   Physical/Chemical

    Biological

6.Test & Reference items

7.Standard operating procedures

8.Performance of Study

   Study Plan

   Conduct of Study

9.Reporting of results

10.Storage of Records and Reports

PHARMACODYNAMICS

Pharmacodynamics is the study of the physiological effects of drugs on the body or on microorganisms or parasites within or on the body and the mechanisms of drug action and the relationship between drug concentration and effect.

Effects on the body

The majority of drugs either

 (a) mimic or inhibit normal physiological/biochemical processes or inhibit pathological processes in animals    (b) inhibit vital processes of endo- or ectoparasites and microbial organisms.

There are 5 main drug actions:

depressing

stimulating

destroying cells (cytotoxicity)

irritation

replacing substancesDesired activity

The desired activity of a drug :

Cellular membrane disruption

Chemical reaction

Interaction with enzyme proteins

Interaction with structural proteins

Interaction with carrier proteins

Interaction with ion channels

Ligand binding to receptors:

Hormone receptors

Neuromodulator receptors

Neurotransmitter receptors

General anesthetics were once thought to work by disordering the neural membranes, thereby altering the Na+ influx. Antacids and chelating agents combine chemically in the body. Enzyme-substrate binding is a way to alter the production or metabolism of key endogenous chemicals, for example aspirin irreversibly inhibits the enzyme prostaglandin synthetase (cyclooxygenase) thereby preventing inflammatory response. Colchicine, a drug for gout, interferes with the function of the structural protein tubulin, while Digitalis, a drug still used in heart failure, inhibits the activity of the carrier molecule, Na-K-ATPase pump. The widest class of drugs act as ligands which bind to receptors which determine cellular effects. Upon drug binding, receptors can elicit their normal action (agonist), blocked action (antagonist), or even action opposite to normal (inverse agonist).In principle, a pharmacologist would aim for a target plasma concentration of the drug for a desired level of response. In reality, there are many factors affecting this goal. Pharmacokinetic factors determine peak concentrations, and concentrations cannot be maintained with absolute consistency because of metabolic breakdown and excretory clearance. Genetic factors may exist which would alter metabolism or drug action itself, and a patient's immediate status may also affect indicated dosage.

Undesirable effects

Undesirable effects of a drug

Increased probability of cell mutation (carcinogenic activity)

A multitude of simultaneous assorted actions which may be deleterious

Interaction (additive, multiplicative, or metabolic)

Induced physiological damage, or abnormal chronic conditions

PHYTOREMEDIATION

Phytoremediation describes the treatment of environmental problems (bioremediation) through the use of plants which mitigate the environmental problem without the need to excavate the contaminant material and dispose of it elsewhere.

The word's etymology comes from the GreekPhytoremediation consists in mitigating pollutant concentrations in contaminated soils, water or air with plants able to contain, degrade or eliminate metals, pesticides, solvents, explosives, crude oil and its derivatives, and various other contaminants, from the media that contain them

Various phytoremediation processes

A range of processes mediated by plants or algae are useful in treating environmental problems:

Phytoextraction - uptake and concentration of substances from the environment into the plant biomass.

Phytostabilization - reducing the mobility of substances in the environment, for example by limiting the leaching of substances from the soil.

Phytotransformation - chemical modification of environmental substances as a direct result of plant metabolism, often resulting in their inactivation, degradation (phytodegradation) or immobilization (phytostabilization).

Phytostimulation - enhancement of soil microbial activity for the degradation of contaminants, typically by organisms that associate with roots. This process is also known as rhizosphere degradation. Phytostimulation can also involve aquatic plants supporting active populations of microbial degraders, as in the stimulation of atrazine degradation by hornwort.

Phytovolatilization - removal of substances from soil or water with release into the air, sometimes as a result of phytotransformation to more volatile and / or less polluting substances.

Rhizofiltration - filtering water through a mass of roots to remove toxic substances or excess nutrients. The pollutants remain absorbed in or adsorbed to the roots.

MICROBIAL EXTRACTION OF PETROLEUM

The extraction of petroleum is the process by which usable petroleum is extracted and removed from the earth

Locating the oil field

Nowadays, the geologists use seismic surveys to search for geological structures that may form oil reservoirs. The "classic" method includes making underground explosion nearby and observing the seismic response that provides information about the geological structures under the ground . However, "passive" methods that extract information from naturally-occurring seismic waves are also known .Other instruments such as gravimeters and magnetometers are also sometimes used in the search for petroleum. When extracting crude oil, it normally starts by drilling wells into the underground reservoir. When an oil well has been tapped, a geologist (known on the rig as the "mudlogger") will note its presence. Historically, in the USA, some oil fields existed where the oil rose naturally to the surface, but most of these fields have long since been used up, except certain places in Alaska. Often many wells (called multilateral wells) are drilled into the same reservoir, to ensure that the extraction rate will be economically viable. Also, some wells (secondary wells) may be used to pump water, steam, acids or various gas mixtures into the reservoir to raise or maintain the reservoir pressure, and so maintain an economic extraction rate.

Oil extraction and recovery

Primary recovery

During the primary recovery stage, reservoir drive comes from a number of natural mechanisms. These include: natural water displacing oil downward into the well, expansion of the natural gas at the top of the reservoir, expansion of gas initially dissolved in the crude oil, and gravity drainage resulting from the movement of oil within the reservoir from the upper to the lower parts where the wells are located. Recovery factor during the primary recovery stage is typically 5-15%.While the underground pressure in the oil reservoir is sufficient to force the oil to the surface, all that is necessary is to place a complex arrangement of valves (the Christmas tree) on the well head to connect the well to a pipeline network for storage and processing.

Secondary recovery

Over the lifetime of the well the pressure will fall, and at some point there will be insufficient underground pressure to force the oil to the surface. After natural reservoir drive diminishes, secondary recovery methods are applied. They rely on the supply of external energy into the reservoir in the form of injecting fluids to increase reservoir pressure, hence replacing or increasing the natural reservoir drive with an artificial drive. Sometimes pumps, such as beam pumps and electrical submersible pumps (ESPs), are used to bring the oil to the surface. Other secondary recovery techniques increase the reservoir's pressure by water injection, natural gas reinjection and gas lift, which injects air, carbon dioxide or some other gas into the bottom of a production well, reducing the overall density of fluid in the wellbore. Typical recovery factor from water-flood operations is about 30%, depending on the properties of oil and the characteristics of the reservoir rock. On average, the recovery factor after primary and secondary oil recovery operations is between 30 and 50%.

Tertiary recovery

Steam is injected into many oil fields where the oil is thicker and heavier than normal crude oilTertiary, or enhanced oil recovery methods increase the mobility of the oil in order to increase production.Thermally enhanced oil recovery methods (TEOR) are tertiary recovery techniques that heat the oil, thus reducing its viscosity and making it easier to extract. Steam injection is the most common form of TEOR, and is often done with a cogeneration plant. In this type of cogeneration plant, a gas turbine is used to generate electricity and the waste heat is used to produce steam, which is then injected into the reservoir. This form of recovery is used extensively to increase oil production in the San Joaquin Valley, which has very heavy oil, yet accounts for 10% of the United States' oil production.[citation needed] In-situ burning is another form of TEOR, but instead of steam, some of the oil is burned to heat the surrounding oil.Occasionally, surfactants (detergents) are injected to alter the surface tension between the water and oil in the reservoir, mobilizing oil which would otherwise remain in the reservoir as residual oil.Another method to reduce viscosity is carbon dioxide flooding.Tertiary recovery allows another 5% to 15% of the reservoir's oil to be recovered.

Tertiary recovery begins when secondary oil recovery isn't enough to continue adequate production, but only when the oil can still be extracted profitably. This depends on the cost of the extraction method and the current price of crude oil. When prices are high, previously unprofitable wells are brought back into production and when they are low, production is curtailed.

Microbial treatments is another tertiary recovery method. Special blends of the microbes are used to treat and break down the hydrocarbon chain in oil thus making the oil easy to recover as well as being more economic versus other conventional methods. In some states, such as Texas, there are tax incentives for using these microbes in what is called a secondary tertiary recovery. Very few companies supply these, however companies like Bio Tech, Inc. have proven very successful in waterfloods across Texas.

MICROBIAL FUEL CELL

A microbial fuel cell (MFC) or biological fuel cell is a bio-electrochemical system that drives a current by mimicking bacterial interactions found in nature. Mediator-less MFCs are a much more recent development and due to this the factors that affect optimum operation, such as the bacteria used in the system, the type of ion membrane, and the system conditions such as temperature, are not particularly well understood. Bacteria in mediator-less MFCs typically have electrochemically-active redox enzymes such as cytochromes on their outer membrane that can transfer electrons to external materials

Microbial fuel cell

A microbial fuel cell is a device that converts chemical energy to electrical energy by the catalytic reaction of microorganisms (Allen and Bennetto, 1993). A typical microbial fuel cell consists of anode and cathode compartments separated by a cation (positively charged ion) specific membrane. In the anode compartment, fuel is oxidized by microorganisms, generating electrons and protons. Electrons are transferred to the cathode compartment through an external electric circuit, and the protons are transferred to the cathode compartment through the membrane. Electrons and protons are consumed in the cathode compartment, combining with oxygen to form water. In general, there are two types of microbial fuel cells: mediator and mediator-less microbial fuel cells.

Mediator Microbial Fuel Cell

Most of the microbial cells are electrochemically inactive. The electron transfer from microbial cells to the electrode is facilitated by mediators such as thionine, methyl viologen, methyl blue, humic acid, neutral red and so on (Delaney et al., 1984; Lithgow et al., 1986). Most of the mediators available are expensive and toxic.

BIOFERTILIZERS

Biofertilizer is a substance which contains living microorganisms which, when applied to seed, plant surfaces, or soil, colonizes the rhizosphere or the interior of the plant and promotes growth by increasing the supply or availability of primary nutrients to the host plant. Biofertilizers add nutrients through the natural processes of Nitrogen fixation , solubilizing phosphorus, and stimulating plant growth through the synthesis of growth promoting substances. Biofertilizers can be expected to reduce the use of chemical fertilizers and pesticides. The microorganisms in biofertilizers restore the soil's natural nutrient cycle and build soil organic matter. Through the use of biofertilizers, healthy plants can be grown while enhancing the sustainability and the health of soil. Since they play several roles, a preferred scientific term for such beneficial bacteria is plant-growth promoting rhizobacteria (PGPR]. Therefore, they are extremely advantageous in enriching the soil fertility and fulfilling the plant nutrient requirements by supplying the organic nutrients through microorganism and their byproduct. Hence, biofertilizers do not contain any chemicals which are harmful to the living soil. Biofertilizers are Eco-friendly organic agro-input and more cost effective than chemical fertilizers. Biofertilizers like Rhizobium, Azotobacter, Azospirillum and blue green algae (BGA) are in use since long time ago. Rhizobiuminoculant is used for leguminous crops. Azotobacter can be used with crops like wheat, maize, mustard, cotton, potato and other vegetable crops. Azospirillum inoculants are recommended mainly for sorghum, millets, maize, sugarcane and wheat. Blue green algae belonging to genera Nostoc, Anabaena, Tolypothrix and Aulosira fix atmospheric nitrogen and are used as inoculants for paddy crop grown both under upland and low land conditions. Anabaena in association with water fern Azolla contributes nitrogen up to 60 Kg/ha/season and also enriches soils with organic matter Other types of bacteria, so-called phosphate solubilizing bacteria like Pantoea agglomerans strain P5, and Pseudomonas putida strain P13 are able to solubilize the insoluble phosphate from organic and inorganic phosphate source  . In fact, due to immobilization of phosphate by mineral ions such as Fe, Al and Ca or organic acids, the rate of available phosphate (Pi) in soil is well below plant needs. In addition, chemical Pi fertilizer are also immobilized in the soil immediately so that less than 20 percent of added fertilizer is absorbed by plants. Therefore, reduction in Pi resources, on one hand, and environmental pollutions resulted from both production and applications of chemical Pi fertilizer, on the other hand, have already demanded the use of new generation of phosphate fertilizers globally known as phosphate solubilizing bacteria or phosphate biofertilizers.

Advantages of Biofertilizers

Cost effective relative to chemical fertilizer and reduces the costs towards fertilizers use, especially regarding nitrogen and phosphorus. It is environmentally friendly fertilizer that not only prevents damaging the natural source but helps to some extend clean the nature from precipitated chemical fertilizer.

BIOLEACHING

Bioleaching is the extraction of specific metals from their ores through the use of bacteria. This is much cleaner than the tradtitional heap leaching using cyanide.[1] Bioleaching is one of several applications within biohydrometallurgy and several methods are used to recover copper, zinc, lead, arsenic, antimony, nickel, molybdenum, gold, and cobalt.

The Process

The extraction of gold from its ore can involve numerous ferrous and sulfur oxidizing bacteria, including Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans (formerly known as Thiobacillus). For example, bacteria catalyse the breakdown of the mineral arsenopyrite (FeAsS) by oxidising the sulfur and metal (in this case arsenic ions) to higher oxidation states whilst reducing dioxygen by H2 and Fe3+. This allows the soluble products to dissolve.

FeAsS(s) → Fe2+(aq) + As3+(aq) + S6+(aq)

This process actually occurs at the cell membrane of the bacteria. The electrons pass into the cells and are used in biochemical processes to produce energy for the bacteria to reduce oxygen molecules to water.

In stage 2, bacteria oxidise Fe2+ to Fe3+ (whilst reducing O2).

Fe2+ → Fe3+

They then oxidise the metal to a higher positive oxidation state. With the electrons gained, they reduce Fe3+ to Fe2+ to continue the cycle.

M3+ → M5+

The gold is now separated from the ore and in solution.

The process for copper is very similar. The mineral chalcopyrite (CuFeS2) follows the two stages of being dissolved and then further oxidised, with Cu2+ ions being left.

SUPEROXIDE DISMUTASE

Superoxide dismutases  are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism

General

Discovered by Irwin Fridovich and Joe McCord, SOD enzymes were previously thought to be several metalloproteins with unknown function (for example, CuZnSOD was known as erythrocuprein). Several common forms of SOD exist: they are proteins cofactored with copper and zinc, or manganese, iron, or nickel. For example, Brewer (1967) identified a protein that became known as superoxide dismutase as an indophenol oxidase by protein analysis of starch gels using the phenazine-tetrazolium technique.

There are three major families of superoxide dismutase, depending on the metal cofactor: Cu/Zn (which binds both copper and zinc), Fe and Mn types (which bind either iron or manganese), and finally the Ni type, which binds nickel.

Copper and zinc – most commonly used by eukaryotes. The cytosols of virtually all eukaryotic cells contain an SOD enzyme with copper and zinc (Cu-Zn-SOD). (For example, Cu-Zn-SOD available commercially is normally purified from the bovine erythrocytes: The Cu-Zn enzyme is a homodimer of molecular weight 32,500. The two subunits are joined primarily by hydrophobic and electrostatic interactions. The ligands of copper and zinc are histidine side-chains.

Iron or manganese – used by prokaryotes and protists

Iron – E. coli and many other bacteria also contain a form of the enzyme with iron (Fe-SOD); some bacteria contain Fe-SOD, others Mn-SOD, and some contain both. (For the E. coli Fe-SOD: . Fe-SOD can be found in the plastids of plants. The active sites of Mn and Fe superoxide dismutases contain the same type of amino acid side-chains.

Manganese – Chicken liver (and nearly all other) mitochondria, and many bacteria (such as E. coli) contain a form with manganese (Mn-SOD). (For example, the Mn-SOD found in a human mitochondrion: The ligands of the manganese ions are 3 histidine side-chains, an aspartate side-chain and a water molecule or hydroxy ligand, depending on the Mn oxidation state (respectively II and III).

nickel – prokaryotic. A hexameric structure built from right-handed 4-helix bundles, each containing N-terminal hooks that chelate a Ni ion. The Ni-hook contains the motif His-Cys-X-X-Pro-Cys-Gly-X-Tyr; it provides most of the interactions critical for metal binding and catalysis and is, therefore, a likely diagnostic of NiSODs.

BIOMINING

Microbes useful in metal recovery


The microbes that are suitable and useful for metal recovery differ depending upon the temperature of the metal recovery process. At 40°C, the most important microbes are Gram negative bacteria belonging to genera Acidithiobacillus and Lactospirillum, which include
 (i) iron and sulphur oxidizing At. ferrooxidans,
(ii) sulphur oxidizing At. thiooxidans and At. caldus, and
 (iii) iron oxidizing L. ferrooxidans and L. ferriphilum.
At 50°C, the suitable biornining microbes include At. caldus, some species of the genus Leptospirillum, and some Gram negative bacteria including the members of the genera Sulphobacillus and Acidimicrobium, and at 70°C, the useful biomining microbes are members of the group archaea (Sulphobolus and Metallophaera). Improvement of bacterial strains for enhanced rate of leaching of metals is also being attempted for several of these bacteria.

DNase

A deoxyribonuclease (DNase, for short) is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. Deoxyribonucleases are thus one type of nuclease. A wide variety of deoxyribonucleases are known, which differ in their substrate specificities, chemical mechanisms, and biological functions.
Modes of action
Some DNases cleave only residues at the ends of DNA molecules (exodeoxyribonucleases, a type of exonuclease). Others cleave anywhere along the chain (endodeoxyribonucleases, a subset of endonucleases).
Some are fairly indiscriminate about the DNA sequence at which they cut, while others, including restriction enzymes, are very sequence-specific.Some cleave only double-stranded DNA, others are specific for single-stranded molecules, and still others are active toward both.DNase enzymes can be inhaled using a nebuliser by cystic fibrosis sufferers. DNase enzymes help because white blood cells accumulate in the mucus and when they breakdown they release DNA which adds to the 'stickiness' of the mucus. DNase enzymes breakdown the DNA and the mucus is much easier to clear from the lungs.
Types of deoxyribonucleases


The two main types of DNase found in metazoans are known as deoxyribonuclease I and deoxyribonuclease II.
Other types of DNase include Micrococcal nuclease

ASPARAGINASE ENZYME

Asparaginase is an enzyme that catalyzes the hydrolysis of asparagine to aspartic acid. It is marketed under the brand name Elspar, to treat acute lymphoblastic leukemia (ALL) and is also used in some mast cell tumor protocols. [1] Unlike other chemotherapy agents, it can be given as an intramuscular, subcutaneous, or intravenous injection without fear of tissue irritation

Mechanism of action

The rationale behind asparaginase is that it takes advantage of the fact that ALL leukemic cells are unable to synthesize the non-essential amino acid asparagine, whereas normal cells are able to make their own asparagine; thus leukemic cells require high amount of asparagine. These leukemic cells depend on circulating asparagine. Asparaginase, however, catalyzes the conversion of L-asparagine to aspartic acid and ammonia. This deprives the leukemic cell of circulating asparagine.

Side effects

The main side-effect is an allergic or hypersensitivity reaction. Asparaginase has also been associated with pancreatitis. Additionally, it can also be associated with a coagulopathy as it decreases protein synthesis, including synthesis of coagulation factors (eg progressive isolated decrease of fibrinogen) and anticoagulant factor (generally antithrombin III; sometimes protein C & S as well), leading to bleeding or thrombotic events such as stroke

SOLAR ENERGY

Solar energy, radiant light and heat from the sun, has been harnessed by humans since ancient times using a range of ever-evolving technologies. Solar radiation, along with secondary solar-powered resources such as wind and wave power, hydroelectricity and biomass, account for most of the available renewable energy on earth. Only a minuscule fraction of the available solar energy is used.Solar powered electrical generation relies on heat engines and photovoltaics. Solar energy's uses are limited only by human ingenuity. A partial list of solar applications includes space heating and cooling through solar architecture, potable water via distillation and disinfection, daylighting, solar hot water, solar cooking, and high temperature process heat for industrial purposes.To harvest the solar energy, the most common way is to use solar panels.

Solar technologies are broadly characterized as either passive solar or active solar depending on the way they capture, convert and distribute solar energy. Active solar techniques include the use of photovoltaic panels and solar thermal collectors to harness the energy. Passive solar techniques include orienting a building to the Sun, selecting materials with favorable thermal mass or light dispersing properties, and designing spaces that naturally circulate air

The Earth receives 174 petawatts (PW) of incoming solar radiation (insolation) at the upper atmosphere.Approximately 30% is reflected back to space while the rest is absorbed by clouds, oceans and land masses. The spectrum of solar light at the Earth's surface is mostly spread across the visible and near-infrared ranges with a small part in the near-ultraviolet.Earth's land surface, oceans and atmosphere absorb solar radiation, and this raises their temperature. Warm air containing evaporated water from the oceans rises, causing atmospheric circulation or convection. When the air reaches a high altitude, where the temperature is low, water vapor condenses into clouds, which rain onto the Earth's surface, completing the water cycle. The latent heat of water condensation amplifies convection, producing atmospheric phenomena such as wind, cyclones and anti-cyclones.Sunlight absorbed by the oceans and land masses keeps the surface at an average temperature of 14 °C. By photosynthesis green plants convert solar energy into chemical energy, which produces food, wood and the biomass from which fossil fuels are derived.

Yearly Solar fluxes & Human Energy Consumption

Solar 3,850,000 EJ

Wind 2,250 EJ

Biomass 3,000 EJ

Primary energy use (2005) 487 EJ

Electricity (2005) 56.7 EJThe total solar energy absorbed by Earth's atmosphere, oceans and land masses is approximately 3,850,000 exajoules (EJ) per year. In 2002, this was more energy in one hour than the world used in one year

BIOFILM

A BIOFILM is an aggregate of microorganisms in which cells adhere to each other and/or to a surface. These adherent cells are frequently embedded within a self-produced matrix of extracellular polymeric substance (EPS). Biofilm EPS, which is also referred to as slime, is a polymeric conglomeration generally composed of extracellular DNA, proteins, and polysaccharides in various configurations. Biofilms may form on living or non-living surfaces, and represent a prevalent mode of microbial life in natural, industrial and hospital settings. The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single-cells that may float or swim in a liquid medium.Microbes form a biofilm in response to many factors, which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics.When a cell switches to the biofilm mode of growth, it undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated.

BIOREMEDIATION

Bioremediation can be defined as any process that uses microorganisms, fungi, green plants or their enzymes to return the natural environment altered by contaminants to its original condition. Bioremediation may be employed to attack specific soil contaminants, such as degradation of chlorinated hydrocarbons by bacteria. An example of a more general approach is the cleanup of oil spills by the addition of nitrate and/or sulfate fertilisers to facilitate the decomposition of crude oil by indigenous or exogenous bacteria.

Overview and Application

Naturally occurring bioremediation and phytoremediation have been used for centuries. For example, desalination of agricultural land by phytoextraction has a long tradition. Bioremediation technology using microorganisms was reportedly invented by George M. Robinson. He was the assistant county petroleum engineer for Santa Maria, California. During the 1960s, he spent his spare time experimenting with dirty jars and various mixes of microbes.Bioremediation technologies can be generally classified as in situ or ex situ. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be treated elsewhere. Some examples of bioremediation technologies are bioventing, landfarming, bioreactor, composting, bioaugmentation, rhizofiltration, and biostimulation.Bioremediation can occur on its own (natural attenuation or intrinsic bioremediation) or can be spurred on via the addition of fertilizers to increase the bioavailability within the medium (biostimulation). Recent advancements have also proven successful via the addition of matched microbe strains to the medium to enhance the resident microbe population's ability to break down contaminants (bioaugmentation).Not all contaminants, however, are easily treated by bioremediation using microorganisms. For example, heavy metals such as cadmium and lead are not readily absorbed or captured by organisms. The assimilation of metals such as mercury into the food chain may worsen matters. Phytoremediation is useful in these circumstances, because natural plants or transgenic plants are able to bioaccumulate these toxins in their above-ground parts, which are then harvested for removal. The heavy metals in the harvested biomass may be further concentrated by incineration or even recycled for industrial use.The elimination of a wide range of pollutants and wastes from the environment requires increasing our understanding of the relative importance of different pathways and regulatory networks to carbon flux in particular environments and for particular compounds and they will certainly accelerate the development of bioremediation technologies and biotransformation processes.

LANDFILL

Disposing of waste in a landfill involves burying the waste, and this remains a common practice in most countries. Landfills were often established in abandoned or unused quarries, mining voids or borrow pits. A properly-designed and well-managed landfill can be a hygienic and relatively inexpensive method of disposing of waste materials. Older, poorly-designed or poorly-managed landfills can create a number of adverse environmental impacts such as wind-blown litter, attraction of vermin, and generation of liquid leachate. Another common byproduct of landfills is gas (mostly composed of methane and carbon dioxide), which is produced as organic waste breaks down anaerobically. This gas can create odor problems, kill surface vegetation, and is a greenhouse gas.A landfill compaction vehicle in action.Design characteristics of a modern landfill include methods to contain leachate such as clay or plastic lining material. Deposited waste is normally compacted to increase its density and stability, and covered to prevent attracting vermin (such as mice or rats). Many landfills also have landfill gas extraction systems installed to extract the landfill gas. Gas is pumped out of the landfill using perforated pipes and flared off or burnt in a gas engine to generate electricity.

WASTE MANAGEMENT

Waste management is the collection, transport, processing, recycling or disposal, and monitoring of waste materials. The term usually relates to materials produced by human activity, and is generally undertaken to reduce their effect on health, the environment or aesthetics. Waste management is also carried out to recover resources from it. Waste management can involve solid, liquid, gaseous or radioactive substances, with different methods and fields of expertise for each. Waste management practices differ for developed and developing nations, for urban and rural areas, and for residential and industrial producers. Management for non-hazardous residential and institutional waste in metropolitan areas is usually the responsibility of local government authorities, while management for non-hazardous commercial and industrial waste is usually the responsibility of the generator.

Energy Recovery
 The energy content of waste products can be harnessed directly by using them as a direct combustion fuel, or indirectly by processing them into another type of fuel. Recycling through thermal treatment ranges from using waste as a fuel source for cooking or heating, to anaerobic digestion and the use of the gas fuel  to fuel for boilers to generate steam and electricity in a turbine. Pyrolysis and gasification are two related forms of thermal treatment where waste materials are heated to high temperatures with limited oxygen availability. The process usually occurs in a sealed vessel under high pressure. Pyrolysis of solid waste converts the material into solid, liquid and gas products. The liquid and gas can be burnt to produce energy or refined into other chenmical products (chemical refinery). The solid residue (char) can be further refined into products such as activated carbon. Gasification and advanced Plasma arc gasification are used to convert organic materials directly into a synthetic gas (syngas) composed of carbon monoxide and hydrogen. The gas is then burnt to produce electricity and steam. An alternative to pyrolisis is high temperature and pressure supercritical water decomposition (hydrothermal monophasic oxidation).

WIND POWER OR WIND ENERGY

Wind power is the conversion of wind energy into a useful form of energy, such as using wind turbines to make electricity, wind mills for mechanical power, wind pumps for pumping water or drainage, or sails to propel ships.At the end of 2009, worldwide nameplate capacity of wind-powered generators was 159.2 gigawatts (GW). Energy production was 340 TWh, which is about 2% of worldwide electricity usage; and is growing rapidly, having doubled in the past three years. Several countries have achieved relatively high levels of wind power penetration (with large governmental subsidies), such as 19% of stationary electricity production in Denmark, 13% in Spain and Portugal, and 7% in Germany and the Republic of Ireland in 2008. As of May 2009, eighty countries around the world are using wind power on a commercial basis. Large-scale wind farms are connected to the electric power transmission network; smaller facilities are used to provide electricity to isolated locations. Utility companies increasingly buy back surplus electricity produced by small domestic turbines. Wind energy as a power source is attractive[says who?] as an alternative to fossil fuels, because it is plentiful, renewable, widely distributed, clean, and produces no greenhouse gas emissions during operation. However, the construction of wind farms is not universally welcomed because of their visual impact and other effects on the environment.Wind power is non-dispatchable, meaning that for economic operation, all of the available output must be taken when it is available. Other resources, such as hydropower, and standard load management techniques must be used to match supply with demand. The intermittency of wind seldom creates problems when using wind power to supply a low proportion of total demand, but costs rise as does the proportion

TIDAL ENERGY

Tidal power, sometimes also called tidal energy, is a form of hydropower that converts the energy of tides into electricity or other useful forms of power. The first large-scale tidal power plant (the Rance Tidal Power Station) started operation in 1966.Although not yet widely used, tidal power has potential for future electricity generation. Tides are more predictable than wind energy and solar power. Among sources of renewable energy, tidal power has traditionally suffered from relatively high cost and limited availability of sites with sufficiently high tidal ranges or flow velocities, thus constricting its total availability. However, many recent technological developments and improvements, both in design (e.g. dynamic tidal power, tidal lagoons) and turbine technology (e.g. new axial turbines, crossflow turbines), are suggesting that the total availability of tidal power may be much higher than previously assumed, and that economic and environmental costs may be brought down to competitive levels. Historically, tide mills have been used, both in Europe and on the Atlantic coast of North America. The earliest occurrences date from the Middle Ages, or even from Roman times. Tidal power is the only form of energy which derives directly from the relative motions of the Earth–Moon system, and to a lesser extent from the Earth–Sun system. The tidal forces produced by the Moon and Sun, in combination with Earth's rotation, are responsible for the generation of the tides. Other sources of energy originate directly or indirectly from the Sun, including fossil fuels, conventional hydroelectric, wind, biofuels, wave power and solar. Nuclear is derived using radioactive material from the Earth, geothermal power uses the Earth's internal heat which comes from a combination of residual heat from planetary accretion (about 20%) and heat produced through radioactive decay (80%)

RENEWABLE ENERGY SOURCE

Renewable energy is energy which comes from natural resources such as sunlight, wind, rain, tides, and geothermal heat, which are renewable (naturally replenished). In 2006, about 18% of global final energy consumption came from renewables, with 13% coming from traditional biomass, which is mainly used for heating, and 3% from hydroelectricity. New renewables (small hydro, modern biomass, wind, solar, geothermal, and biofuels) accounted for another 2.4% and are growing very rapidly. The share of renewables in electricity generation is around 18%, with 15% of global electricity coming from hydroelectricity and 3.4% from new renewables.
  Wind power is growing at the rate of 30% annually, with a worldwide installed capacity of 157,900 megawatts (MW) in 2009, and is widely used in Europe, Asia, and the United States. At the end of 2009, cumulative global photovoltaic (PV) installations surpassed 21,000 MW and PV power stations are popular in Germany and Spain. Solar thermal power stations operate in the USA and Spain, and the largest of these is the 354 MW SEGS power plant in the Mojave Desert. The world's largest geothermal power installation is The Geysers in California, with a rated capacity of 750 MW. Brazil has one of the largest renewable energy programs in the world, involving production of ethanol fuel from sugar cane, and ethanol now provides 18% of the country's automotive fuel. Ethanol fuel is also widely available in the USA.

  While most renewable energy projects and production is large-scale, renewable technologies are also suited to small off-grid applications, sometimes in rural and remote areas, where energy is often crucial in human development. Kenya has the world's highest household solar ownership rate with roughly 30,000 small (20–100 watt) solar power systems sold per year.Climate change concerns, coupled with high oil prices, peak oil, and increasing government support, are driving increasing renewable energy legislation, incentives and commercialization. New government spending, regulation and policies helped the industry weather the 2009 economic crisis better than many other sectors.